Simultaneous determination of substrate and product in the process of preparation of valienamine by capillary zone electrophoresis

✍ Scribed by Xiao-Dong Wei; Wen-Jie Zhao; Min Gu; Bo Zhao; Rong-Ying Yao

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 197 KB
• Volume: 33
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200900813

✦ Synopsis

Abstract

A simple and rapid CZE method was established for the simultaneous determination of valienamine, acarbose and validamycin A, using a 20‐kV CZE with the detection wavelength of 193 nm and 50 mM phosphoric acid–20 mM Tris (pH 5.3) as a running buffer. The calibration curves of valienamine, acarbose, and validamycin A showed a good linear relationship at a concentration range of 5–1000 μg/mL. The detection limits of valienamine, acarbose, and validamycin A were 0.3, 0.6, and 0.6 μg/mL, respectively, and the average recoveries of each of the above were 99.9, 99.5, and 100.3%. The method has been successfully applied for simultaneous determination of substrate and product in the process of preparation of valienamine.