Simultaneous determination of noradrenaline and dopamine in Portulaca oleracea L. by capillary zone electrophoresis

✍ Scribed by Mei-E Yue; Ting-Fu Jiang; Yan-Ping Shi

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 466 KB
• Volume: 28
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200400045

✦ Synopsis

Abstract

A simple and rapid capillary electrophoretic method was developed for the simultaneous determination of noradrenaline (NA) and dopamine (DA) in Portulaca oleracea L__.__ The buffer solution used in this method was 40 mM tris (hydroxymethyl) aminomethane (Tris)‐H~3~PO~4~ at pH 2.00 containing 15% methanol. The effects of pH value, organic modifier, and applied voltage were investigated. The linear ranges of NA and DA were 0.5–100 μg/mL (r = 0.9952) and 6.25–200 μg/mL (r = 0.9992), respectively. The relative standard deviations of the corrected peak area were 6.73% and 4.26%, respectively. NA and DA in Portulaca oleracea L__.__ were simultaneous determined successfully within 5.6 min. In this way, the contents of NA and DA in different parts (stem, leaves, and seeds) of P. oleracea L__.__ and in different extracts of leaves with different solvents (distilled water, 50% methanol, and methanol) were studied.