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Simultaneous determination of nicotine, cotinine and five additional nicotine metabolites in the urine of smokers using pre-column derivatisation and high-performance liquid chromatography

✍ Scribed by Robert D. Barlow; Phillip A. Thompson; Rossana B. stone


Publisher
Elsevier Science
Year
1987
Weight
402 KB
Volume
419
Category
Article
ISSN
0378-4347

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An SPE-LC-MS/MS method was developed, validated and applied to the determination of nicotine and five major metabolites in human urine: cotinine, trans-3'-hydroxycotinine, nicotine-N-glucuronide, cotinine-N-glucuronide and trans-3'-hydroxycotinine-O-glucuronide. A 500 microL urine sample was pH-adju