Simultaneous determination of four tanshinones in salvia miltiorrhiza by pressurized liquid extraction and capillary electrochromatography

✍ Scribed by Peng Li; Shao-Ping Li; Feng-Qing Yang; Yi-Tao Wang

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 796 KB
• Volume: 30
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200600368

✦ Synopsis

Abstract

A pressurized liquid extraction (PLE) and CEC were developed for the simultaneous determination of four tanshinones (dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone IIA) in Salvia miltiorrhiza. High extraction efficiency (> 98.5%) was achieved under the optimum PLE conditions. A good separation was obtained by using a Hypersil C~18~ capillary (3 μm, 100 μm/25 cm) with a mixture of 30 mM Tris‐HCl (pH 8.5)‐ACN (1:3, v/v) as BGE solution running at 20 kV and 20°C within 12 min. All the calibration curves showed good linearity (r^2^ >0.9958) within test ranges. The developed method showed good repeatability for the quantification of four investigated components in S. miltiorrhiza with intra‐ and interday variations of less than 4.4 and 6.8%, respectively. The validated method was successfully applied to quantify four tanshinones in S. miltiorrhiza, which is helpful to control the quality of S. miltiorrhiza.