Simultaneous determination of five tetracycline and macrolide antibiotics in feeds using HPCE

✍ Scribed by Jing Tong; Qinxiong Rao; Kui Zhu; Zhigang Jiang; Shuangyang Ding

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 395 KB
• Volume: 32
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200900415

✦ Synopsis

Abstract

This work demonstrates the potential of HPCE in the analysis of antibiotics in a complex matrix such as feedstuffs. Using 20 mM citric acid–40 mM Na~2~HPO~4~ buffer (pH 2.65), the five antibiotics, tetracycline, oxytetracycline, doxycycline, tilmicosin, and tylosin were successfully separated at 30 kV in a 64.5 cm×75 μm id capillary. Good repeatability, stability, and reliability of the method were supported by <10% CV with mean recoveries of >70%, and the limit of detection of the five analytes was 0.5–1 mg/kg. It was for the first time that a capillary electrophoretic method was employed to simultaneously detect five tetracycline and macrolide antibiotics in animal feeds.