Simultaneous determination of acidic, basic and alcoholic substances relating to monoamine hormones and metabolites in biological material by mass fragmentography (GC/MF)
✍ Scribed by Kobelt, Reinhard ;Wiesener, Günther
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- English
- Weight
- 296 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
Abstract
A method is described for the simultaneous quantitative determination of monoamines and related compounds from urine and brain tissue samples in the pg‐range, using a GC/MF instrument LKB‐2091 provided with a multiple ion detection (MID) system and capillary columns. The simultaneous detection of “fingerprints” with fourteen and more compounds during one single GC run was achieved after the intoduction of “time gates” for the detection of the different characteristic masses. Several modifications to the LKB equipment permitted exact reproducibility of retention times, which is essential for the use of “time gates”. The detection of “fingerprints” with these acidic, basic and alcoholic substances was achieved by the extraction of the dried biological samples with silylating agents.