Simultaneous determination of 5′-deoxy-5-fluorouridine, 5-fluorouracil and 5,6-dihydro-5-fluorouracil in serum by liquid chromatography with diode array UV detection

✍ Scribed by Guerrieri; F. Palmisano; C.G. Zambonin; M. De Lena

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 688 KB
• Volume: 296
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)85148-4

✦ Synopsis

Dihydro-5-fluorouracluorouracil(5-lWH~) , the maiu catabolite of the anticancer drug 54uorouraeil( WU), can be quantitated iu human serum simultaueously with the pro-drug 5'-deoxy-5-f (5'dFWR), the parent drug 5-PU and the aaabolites 5-fluorouridine and 2'-deoxy-5-f by gradient elution-reversed phase liquid chromatography coupled to diode array UV detection. The analytes were extra&d (after protein precipitation with ammouium sulphate) with ethyl acetate-isopropauol (90~10, v/v). The average recovery for 5-PUH, was 78.0% at 0.5 &ml level. 5-PUHz response was linear over three concentration decades and its detection limit was 200 ng/ml of serum. The aualytical procedure has been used for simultaaeous monitoring of 5'-dFIJR, 5-PU and 5-PUHz levels in the serum of a patient under 5'dPUR chemotherapy. Keywomk Liquid chromatography; Fluoropyrimidine dnqp orotnidine-5'-monophosphate (2'-dPUMP) which inhibits DNA synthesis by blocking the thymidilate synthetase system. The alternative anabolic pathway