Simultaneous determination of 11 saponins in Panax notoginseng using HPLC-ELSD and pressurized liquid extraction

✍ Scribed by Jian-Bo Wan; Peng Li; Shaoping Li; Yitao Wang; Tina Ting-Xia Dong; Karl Wah-Keung Tsim

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 475 KB
• Volume: 29
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200600103

✦ Synopsis

Simultaneous determination of 11 saponins in Panax notoginseng using HPLC-ELSD and pressurized liquid extraction

A new HPLC coupled with evaporative light scattering detection (ELSD) method was developed for simultaneous determination of 11 major triterpene saponins, namely notoginsenoside R1 (1),ginsenosides Rg1 (2),Re (3),Rf (4),Rb1 (5),Rg2 (6),Rc (7),Rb2 (8),Rb3 (9), Rd (10), and Rg3 (11) in Panax notoginseng, a commonly used traditional Chinese medicine (TCM). Pressurized liquid extraction (PLE) was employed for sample preparation, and the analysis was achieved using a Zorbax ODS C 18 column eluted with gradient water-ACN in 60 min. The drift tube temperature of ELSD was set at 608C, and nitrogen flowrate was at 1.4 L/min. The method provided good repeatability and sensitivity for quantification of 11 saponins with overall precision (including intra-and interday) and LOD of less than 2.9% (RSD) and 98 ng, respectively. The validated method was successfully applied to quantify 11 saponins in 28 samples of P. notoginseng collected in different places, which is helpful to control the quality of P. notoginseng and its related products.