Simultaneous determination by UPLC–ESI-MS of scoparone, capillarisin, rhein, and emodin in rat urine after oral administration of Yin Chen Hao Tang preparation
✍ Scribed by Haitao Lv; Hui Sun; Xijun Wang; Wenjun Sun; Guozheng Jiao; Dixin Zhou; Li Zhao; Hongxin Cao; Guangmei Zhang
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 936 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1615-9306
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
A simple, sensitive, and validated method was developed for simultaneous determination of scoparone, capillarisin, rhein, and emodin in rat urine by ultra‐performance liquid chromatography/electrospray ionization quadruple time‐of‐flight mass spectrometry (UPLC–MS). The urinary samples were analyzed on an Acquity UPLC BEH C~18~ 1.7 μm 2.1×50 mm column. Scoparone, capillarisin, rhein, and emodin in rat urine were simultaneously analyzed with good separation. The lower limits of detection were 6.0, 9.0, 7.0, and 3.0 ng/mL, and the lower limits of quantification were 20.0, 33.0, 24.0, and 12.0 ng/mL for scoparone, capillarisin, rhein, and emodin, respectively. The intra‐ and inter‐day precisions (RSD) were less than 9%. The intra‐ and inter‐accuracies were found to be in the range of 94.14–104.54% for scoparone, 101.72–107.34% for capillarisin, 95.24–103.59% for rhein, and 101.32–107.82% for emodin at three concentration levels. The absolute recoveries for scoparone, capillarisin, rhein, and emodin were not less than 77.0%. The developed method has been applied to determine scoparone, capillarisin, rhein, and emodin in rat urine after oral administration of Yin Chen Hao Tang preparation, a traditional Chinese medicine formulation widely used in China for treatment of jaundice and liver disorders.