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Simultaneous detection of multiple green fluorescent proteins in live cells by fluorescence lifetime imaging microscopy

✍ Scribed by Rainer Pepperkok; Anthony Squire; Stephan Geley; Philippe I.H. Bastiaens

Book ID
114321464
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
114 KB
Volume
9
Category
Article
ISSN
0960-9822

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## Abstract To accurately interpret the data from fluorescent proteins as reporters of gene activation within living cells, it is important to understand the kinetics of the degradation of the reporter proteins. We examined the degradation kinetics over a large number (>1,000) of single, living cel