## Abstract Essential tremor (ET) is a most common human movement disorder of unknown etiology. Previous reports have shown that the C677T polymorphism of methylenetetrahydrofolate reductase gene has been associated with neurodegenerative disorders. To investigate the role of methylenetetrahydrofol
Simultaneous Detection of Methylenetetrahydrofolate Reductase Gene Polymorphisms, C677T and A1298C, by Melting Curve Analysis with LightCycler
โ Scribed by Sakurako Nakamura; Tsutomu Aoshima; Mayumi Ikeda; Yoshitaka Sekido; Kaoru Shimokata; Toshimitsu Niwa
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 55 KB
- Volume
- 306
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Methylenetetrahydrofolate reductase (MTHFR) is involved in the one-carbon cycle, which is of importance for nucleotide synthesis and methylation of DNA, membranes, proteins and lipids. The MTHFR gene includes two common polymorphisms (rs1801133 or C677T; rs1801131 or A1298C) which both alter enzyme
A microvolume fluorimeter integrated with a rapid thermal cycler allows both amplification and point mutation detection from genomic DNA in approximately 30 min. This homogeneous method combines rapid cycle DNA amplification with allele-specific fluorescent probe melting profiles for product genotyp