Simultaneous detection and quantitation of O6-monoacetylmorphine, morphine and codeine in urine by gas chromatography with nitrogen specific and/or flame ionization detection

✍ Scribed by Trinh Vu-Duc; André Vernay

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 466 KB
• Volume: 4
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1130040206

✦ Synopsis

It is of importance to differentiate heroin intake from the absorption of opiate-containing pharmaceuticals or opiates from other sources. A method for the routine determination of 06-monoacetylmorphine (6-MAM). the specific metabolite of heroin in human urine, by gas chromatography and classical detectors without having recourse to gas chromatography/mass spectrometry-selected ion mode (GUMS-SIM) is described. With dual detection by nitrogen selective and flame ionization detectors, the limits of detection for 6-MAM were determined to be 2 ng/mL and 4 ng/mL urine for a 10 mL sample. When applied to urines preliminarily screened for opiates, the results appeared consistent in comparison with those obtained by GUMS-SIM. The method was also developed for the simultaneous quantitative analysis of morphine and codeine. The linearity was tested up to 600 ng/mL for the three compounds of interest 6-MAM, morphine and codeine and their absolute recoveries were 76%, 78%, 75% respectively.

BIT (BSTFA+TMCS, 99: 1 v/v, Supelco, Bellefonte, PA, USA). Phosphate buffer pH 5.5 was prepared with Na2HP04 0.067 M+KH,PO, 0.067 M (3.9:96.1). Alkaline @ Heyden & Son Limited, 1990