✦ LIBER ✦

Simultaneous demonstration of the Philadelphia chromosome in T, B, and myeloid cells

✍ Scribed by Archie A. Mackinney Jr.; Steven S. Clark; Wayne Borcherding; Marco Fizzotti; Richard Hong

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 516 KB
Volume: 44
Category: Article
ISSN: 0361-8609
DOI: 10.1002/ajh.2830440110

No coin nor oath required. For personal study only.

✦ Synopsis

A patient presented with lymphoblastic lymphoma in lymph-nodes and chronic myelogenous leukemia (CML) in marrow and peripheral blood. All marrow and unstimulated peripheral blood cells contained the Philadelphia chromosome{t(9:22)}. Lymphoma cells were analyzed by flow cytometry and were identified as T cells (CD2+CD5+CD7+CD34+). All fresh lymphoma cells contained the t(9:22) translocation. Cultures of purified peripheral blood T and B cells and specifically stimulated NK cells revealed that 59% of the B cells, 10% of the NK cells, and none of the normal T cells contained the translocation. The lack of translocation in normal peripheral T cells is attributed to their long lifespan. No rearrangement of immunoglobulin or T cell receptor beta or gamma genes was found in either the leukemia or lymphoma cells. Analysis of the DNA from cryopreserved lymphoma biopsy showed clonal rearrangement within the common breakpoint cluster region of the bcr gene identical to the bcr rearrangement in DNA from leukemia blood cells.

The data support the concept that T and B cells originate in the patient's totipotent stem cell from which the CML is also derived.

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