Simultaneous Quantification of Arachidon
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Esther C. Kempen; Peiying Yang; Edward Felix; Timothy Madden; Robert A. Newman
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Article
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2001
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Elsevier Science
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English
โ 122 KB
A validated method is described for the simultaneous analysis of PGE 2 , 11-, 12-, and 5-HETEs from cultured cells using HPLC negative electrospray ionization tandem mass spectrometry (LC/MS/MS). This method permits quantification of selected individual arachidonic acid metabolites from cell extract