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Simultaneous AFM Manipulation and Fluorescence Imaging of Single DNA Strands

✍ Scribed by Andrew Hards; Chunqing Zhou; Markus Seitz; Christoph Bräuchle; Andreas Zumbusch

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
229 KB
Volume
6
Category
Article
ISSN
1439-4235

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✦ Synopsis

Abstract

We report combined atomic force and far‐field fluorescence microscopic experiments which allow the simultaneous atomic force manipulation and optical observation of individual dye‐labeled DNA molecules. A detailed understanding of the binding properties of DNA to different transparent surfaces is prerequisite for these investigations. Atomic force spectroscopy and fluorescence microscopy of single DNA strands yielded detailed insight into two different types of DNA binding onto transparent polylysine‐coated and silanized glass surfaces. We subsequently demonstrate how the different binding can be exploited to perform two types of nanomanipulation experiments: On polylysine, strong electrostatic interactions over the whole length of the DNA strand enable the writing of micrometer‐sized patterns. By contrast, the strong pointwise attachment of DNA to silanized surfaces allows horizontal stretching of single DNA strands to lengths exceeding 1.6 times the contour length of the DNA strand. With this new approach it is possible to directly observe the rupture of the strongly bonded DNA strand.

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