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Simultaeous detection of β-galactosidase activity and surface antigen expression in viable haematopoietic cells

✍ Scribed by Christoph N. Berger; Seong-Seng Tan; Karin S. Sturm

Book ID
102814672
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
893 KB
Volume
17
Category
Article
ISSN
0196-4763

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The b-galactosidase activity encoded by the lacZ gene of Escherichia coli is widely used to monitor successful expression of transfected genes. Fluorogenic substrates allow detection of enzyme activity in viable cells, which, subsequently, can be selected for further study on the basis of fluorescen