Simplified method of identifying severe combined immunodeficient (SCID) mice versus non-SCID mice by flow cytometric analysis of peripheral blood
✍ Scribed by Susan Murray; Sandhya Subramanian; Dennis N. Bourdette; Richard E. Jones
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 142 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
✦ Synopsis
Several studies have utilized simple breeding strategies to create new immunodeficient mouse strains from severe combined immunodeficient (SCID) mice and non-SCID mice with secondary traits in order to evaluate the involvement of lymphocytes and immune responses in a variety of processes. We utilized a breeding strategy with C.B-17scid/scid (SCID) (H-2 d ) mice and SJL (H-2 s ) mice to generate immunodeficient mice that were histocompatible with the inbred SJL strain (H-2 s ) in order to evaluate the role of histocompatible recipient lymphocytes in adoptively transferred autoimmune disease mediated by SJL T lymphocytes. [SCID ؋ SJL]F1 mice (heterozygous for H-2 loci and heterozygous for the SCID mutation) were backcrossed with SCID mice and the resulting offspring expressed a variety of phenotypes, including SCID or non-SCID and H-2 s /H-2 d or H-2 d /H-2 d . In order to screen offspring for the desired phenotype (SCID, H-2 s ), a flow cytometric method utilizing forward-and side-scatter parameters of peripheral blood cells was used to distinguish SCID from non-SCID animals. This method simplified the screening process and was as reliable as anti-CD3 fluorescent monoclonal antibody staining for detecting the presence (non-SCID) or absence (SCID) of T lymphocytes in peripheral blood.