Simplification of two-dimensional NOE spectra of proteins by 13C labeling
β Scribed by Ad Bax; Michael A Weiss
- Publisher
- Elsevier Science
- Year
- 1987
- Weight
- 300 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0022-2364
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## Abstract A new combination of 1D HOHAHA and INEPT pulse sequences is proposed and tested on an antibiotic, amikacin disulphate. The sequence provides ^13^C NMR spectra of chosen structural subunits and thus facilitates the NMR analysis of oligomers.
As aromatic residues very often are part of the hydrophobic essential for an accurate and precise structure determination. core of proteins, the unambiguous assignment of the aromatic Therefore, methods for the unambiguous assignment of aroproton resonances is essential for an accurate and precise s