Simple technique for the preparation of silicone gel particles: The effect of silicone gel particles on oxidative responses of macrophages
✍ Scribed by Azizsoltani, E. ;Myrvik, Q. N. ;Foster, L. A. ;Giridhar, G. ;Gristina, A. G.
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 524 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
A simple technique was developed to prepare phagocytosable-size particles from the silicone gel used in breast implants. Sonication of silicone gel (1 g) in 5 ml of 20 mM sodium phosphate buffer (pH 7.2) containing 1% (wt/vol) polyoxypropylene-polyethylene block surfactant (F-68 or F-108) produced silicone gel particles ranging from 1-50 pm in diameter. Passage of the suspension through a series of filters yielded phagocytosable particles (1-5 pm in diameter) at a concentration of ca. 2 x lo9 particles/ml. The particles remained as individual particles, did not coalesce to form large clumps, and were not pelleted by centrifugation (2000 x g, 20 min). They were not toxic for rabbit alveolar macrophages (AM) during 24 h of incubation at 37"C, did not elicit an oxidative burst from AM in vitro in a luminolenhanced chemiluminescent assay, and did not significantly increase the phorbol myristate acetate (PMA)-elicited oxidative burst by AM. AM isolated from rabbits 2 days after the intravenous injection of silicone particles were not primed or activated (i.e., the AM did not show an enhanced oxidative burst when elicited with PMA in vitro). However, AM isolated from rabbits 2 days after intratracheal injection of the particles were primed but only exhibited a 4-6-fold increase in the oxidative burst elicited with PMA.