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Simple procedure for discrimination and typing of enteric adenoviruses after detection by polymerase chain reaction

✍ Scribed by Annika Allard; Adriana Kajon; Göran Wadell

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
567 KB
Volume
44
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

A procedure was developed for specific discrimination and typing of the enteric human adenoviruses, Ad40 and Ad41, after polymerase chain reaction (PCR) amplification of a sequence in the hexon gene highly conserved among all 47 sero‐types recognised. By Taq I restriction of the 300 bp amplimers, subgenus F DNA could be discriminated from DNAs of adenoviruses belonging to all other subgenera. Discrimination between Ad40 and Ad41 was subsequently achieved by cleavage with either Cfo I, HinP I, Mae III, Mvn I, and/or Rsa I. Thus, PCR detection of viral DNA combined with restriction analysis of amplified products provides a valuable tool for use in epidemiological studies of diarrhoea of adenoviral aetiology. © 1994 Wiley‐Liss, inc.

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