## Cells res&tant to methotrexate (LI210/R7A) and possessing an increased level of dihydrofolate reductase due to gene amplification can be detected by the technique of flow cytofluorimetry using a new fluorescent derivative of methotrexate (F-MTX) based on a putrescine linker. Comparative studies o
Simple Patterning of Cells on a Biocompatible Nonchemically Amplified Resist
โ Scribed by Jin-Baek Kim; Ramakrishnan Ganesan; So Young Yoo; Jae-Hak Choi; Sang Yup Lee
- Book ID
- 102493397
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 148 KB
- Volume
- 27
- Category
- Article
- ISSN
- 1022-1336
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โฆ Synopsis
Abstract
Summary: A simple lithographic process in conjunction with a novel biocompatible nonchemically amplified photoresist material was successfully used for cell patterning. UV light irradiation on selected regions of the nonchemically amplified resist film renders the exposed regions hydrophilic by the formation of carboxylic groups. Mouse fibroblast cells were found to be preferentially aligned and proliferated on the UV light exposed regions of the nonchemically amplified resist film where carboxylic groups were present.
Schematic representation of the simplified lithographic process used for cell patterning.
magnified imageSchematic representation of the simplified lithographic process used for cell patterning.
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