Simple microradiometric technique for the rapid measurement of monoamine oxidase activity in rat testicular minced and teased-tubular preparations
✍ Scribed by Ronald L. Urry; August W. Jaussi; LeGrande C. Ellis
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 511 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Monoamine
oxidase (MAO, monoamine: oxygen oxidoreductase (deaminating), EC 1.4.3.4) activity has been demonstrated in many vertebrate and invertebrate tissues (l-4). Several quantitative methods have been developed to determine the activity of this enzyme in various tissue extracts (5-7) using different substrates. One substrate commonly used in MAO determinations is 5-hydroxytryptamine (5HT) where 5-HT (5,6) is converted into 5-hydroxyindoleacetic acid (5-HIAA) . Different investigations have shown (2,8,9) that MAO is present in the male sex organs and that small amounts of endogenous 5-HT (60-106 nanograms/gram tissue) are present in rat testes (10,ll). Large doses of 5-HT, when injected into adult male rats, caused deleterious effects in their gonads that were evidenced by a reduction in testicular weight and altered spermatogenesis (12-15).
The following investigations were undertaken in order to develop a simple rapid method for the quantitative assay of MAO activity in various testicular preparations so that the enzyme activity could be related to physiological and pathological states of the testis.
Methods
Testicular tissue was obtained from adult male rats (Holtzman strain-12 weeks of age) that were maintained under controlled conditions in