✦ LIBER ✦

Simple Method for Simultaneous Determination of Halothane, Enflurane, and Isoflurane in Krebs Solution Using Capillary Gas Chromatography

✍ Scribed by Don Farthing; Lynne Gehr; Brian Berger; Itaf Fakhry; Domenic Sica

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 161 KB
Volume: 11
Category: Article
ISSN: 0269-3879
DOI: 10.1002/(sici)1099-0801(199701)11:1<29::aid-bmc628>3.0.co;2-c

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✦ Synopsis

A simple gas chromatography (GC) method for the simultaneous determination of halothane, enflurane, and isoflurane in Krebs buffer solution has been developed. The method utilizes methylene chloride as the internal standard and liquid-liquid extraction using chloroform as the solvent. The method demonstrated excellent recovery (100%) of each component and a linear calibration range of 100-700,100-800, and 300-1400 &mL for halothane, isoflurane, and enflurane, respectively. Intra-day accuracy and precision had an error and coefficient of variation of less than 5.1% and 2.7%, respectively.

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