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Simple method for generation of dymanic pH gradient in capillary zone electrophoresis

✍ Scribed by Vladimír Šutáček; František Foret; Petr Boček

Book ID
104145674
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
351 KB
Volume
480
Category
Article
ISSN
1873-3778

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✦ Synopsis

A dynamic pH gradient extends the separation power of zone electrophoresis to mixtures of substances with widely differing pK values. A simple method for the generation of dynamic changes in the pH of the background electrolyte in the separation capillary is described. It is based on a controlled modification of the composition and, thus, of the pH of an electrolyte in the electrode chamber at the injection end of the separation capillary during the analysis. The higher the concentration of H+ in this electrode chamber, the higher is the electromigration flow of H+ into the capillary and the lower is the pH of the actual background electrolyte therein. The utilization of this principle is exemplified by the separation of eleven purine and pyrimidine bases with pK values ranging from 6.0 to 1.9.

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