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Simple Autofocusing System for Single-Molecule FRET Experiment Based on Single-Molecule Image Analysis

โœ Scribed by Hwang, Wonseok; Bae, Sangsu; Hohng, Sungchol


Book ID
121887375
Publisher
Biophysical Society
Year
2013
Tongue
English
Weight
64 KB
Volume
104
Category
Article
ISSN
0006-3495

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## Abstract ## Background Previous reports have demonstrated accurate DNA fragment sizing of linear DNA fragments, from 564 to โ‰ˆ4 ร— 10^5^ bp, in a flow system. Bโ€phycoerythrin (Bโ€PE), commonly used in conventional cytometric applications that require highโ€sensitivity, was the first fluorophore det