✦ LIBER ✦
Simple assay of cyclic nucleotide phosphodiesterase using 32Phosphorus labeled nucleotide; Application to kidney subcellular fractions
✍ Scribed by J.A. Eisman; T.J. Martin
- Book ID
- 102982590
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 598 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A method is described for the estimation of cyclic nucleotide phosphodiesterase activity using ~*P~ospho~s-~abeIed cyclic nucleotides and separation of substrates from reaction products in a single-stage elution on dry neutral alumina columns. The method was used to study tiie subcellular distribution of cyclic AMP and cyclic GMP phosphodiesterase activity in rat renal cortex.