Simple and rapid high-pressure liquid chromatographic determination of papaverine in plasma

✍ Scribed by Steven L. Pierson; John J. Hanigan; Richard E. Taylor; James E. McClurg

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 237 KB
• Volume: 68
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600681224

✦ Synopsis

A rapid and sensitive high-pressure liquid chromatographic (HPLC) assay is described for the quantification of papaverine in plasma. A paired-ion, reversed-phase system was employed with detection at 254 nm. This procedure yielded reproducible results with a sensitivity of 2 ng/ml. Total extraction and quantification time was less than 1 hr. The suitability of this procedure for plasma sample analysis from a bioavailability study was demonstrated.