A novel method for three-dimensional obs
✍
Hisashi Hashimoto; Moriaki Kusakabe; Hiroshi Ishikawa
📂
Article
📅
2007
🏛
John Wiley and Sons
🌐
English
⚖ 630 KB
## Abstract A novel method for acquiring serial images suitable for three‐dimensional reconstruction of vascular networks in the whole brain of mouse was developed. The brain infused with a White India ink‐gelatin solution was fixed and embedded in paraffin containing Sudan Black B through xylene a