✦ LIBER ✦
Simple and Efficient Method for Isolation and Measurement of Cyanobacterial Hepatotoxins by Plant Tests (Sinapis alba L.)
✍ Scribed by P. Kos; G. Gorzo; G. Suranyi; G. Borbely
- Book ID
- 102561070
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 375 KB
- Volume
- 225
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A simple and cost-effective method for isolating and assaying microcystins, cyanobacterial toxins, by C-18 cartridges, DEAE-cellulose (DE-52) chromatography, and a mustard (Sinapis alba L.) plant seedling test is described. The procedure results in a purity of up to (95-) (\mathbf{9 7 %}) microcystin without the need for an HPLC system and justifies the use of the (S). alba L. seedling test in the quantitative assessment of the toxin with an (\mathrm{IC}_{50}) of 3 (\mu \mathrm{g} \mathrm{ml}^{-1}) instead of the mouse intraperitoneal test. 1995 Academic Press. Inc.