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Simple and cost-effective isolation of monocytes from buffy coats

✍ Scribed by Urška Repnik; Miomir Knezevic; Matjaz Jeras

Book ID
114051380
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
752 KB
Volume
278
Category
Article
ISSN
0022-1759

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Monocytes (MOs) are the most commonly used precursors for the generation of dendritic cells (DCs) in vitro. Continuous counter-flow elutriation represents a promising tool to isolate MOs from white blood cell (WBC) products. Thirty whole blood-derived, AB0-identical buffy coats (BCs) were pooled usi