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Simple adaptation of a method measuring the binding of radiolabelled ligands to receptors

โœ Scribed by N.M. Mackenzie

Book ID
118899334
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
99 KB
Volume
67
Category
Article
ISSN
0022-1759

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A simple and rapid assay for measuring radiolabeled ligand binding to purified plasma membranes
โœ William M. Mackin; Chi-Kuang Huang; Barbara-Jean Bormann; Elmer L. Becker ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 716 KB

A simple, rapid assay for measuring radiolabeled ligand binding to purified plasma membranes was developed. In this assay, membrane proteins and ligand are mixed atop a nonmiscible silicone oil (density = 1.029 g/cm9 and incubated to establish equilibrium. The membrane proteins and bound ligand are