Similarity in the metabolic profile in macroscopically involved and un-involved colonic mucosa in patients with inflammatory bowel disease: an in vitro proton (1H) MR spectroscopy study

✍ Scribed by Uma Sharma; Rajiv R. Singh; Vineet Ahuja; Govind K. Makharia; Naranamangalam R. Jagannathan

• Publisher: Elsevier Science
• Year: 2010
• Tongue: English
• Weight: 772 KB
• Volume: 28
• Category: Article
• ISSN: 0730-725X
• DOI: 10.1016/j.mri.2010.03.039

✦ Synopsis

Background:

The histological extent of inflammatory bowel disease (ibd) is greater than that evident by colonoscopic evaluation. we hypothesized that metabolic profile in macroscopically un-involved colonic mucosa in ibd is similar to that of controls with healthy colon. we thus assessed the differences in metabolic profile in macroscopically involved and un-involved colonic mucosa of ibd patients to further substantiate the extent of disease.

Patients and methods:

Colonic mucosal biopsies were obtained and snap frozen from both the macroscopically un-involved and involved colonic mucosa of ibd patients and macroscopically normal colonic mucosa of controls and were subjected to in-vitro high-resolution proton ((1)h) magnetic resonance (mr) spectroscopy and the concentrations of metabolites were determined.

Results:

Thirty-two metabolites were assigned in the proton mr spectrum of colonic mucosa of ibd patients. the concentrations of amino acids (isoleucine, leucine, valine, arginine, lysine, glutamine/glutamate, alanine), membrane metabolites (choline, glycerophosphorylcholine/phosphorylcholine), glycolytic product (lactate) and short chain fatty acid (formate) were significantly lower while significantly high level of glucose were observed in the macroscopically un-involved colonic mucosa of ibd patients compared to the macroscopically normal mucosa of controls. there was no significant difference in the concentrations of metabolites in macroscopically involved and un-involved colonic mucosa of ibd patients.

Conclusions:

The metabolic profile in macroscopically un-involved colonic mucosa of ibd patients is similar to that of macroscopically involved mucosa but different from colonic mucosa of controls. this suggests that even macroscopically un-involved colonic mucosa is metabolically abnormal and may explain the increase in extent of disease with time.