𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Silver ion-induced suicidal erythrocyte death

✍ Scribed by Mentor Sopjani; Michael Föller; Judith Haendeler; Friedrich Götz; Florian Lang

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
256 KB
Volume
29
Category
Article
ISSN
0260-437X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Owing to its antibiotic activity, silver is used for water purification, wound care and a wide variety of implants. Silver metal and silver compounds ionize in solution, and silver ions interfere with the function of a wide variety of proteins. In mammalian cells, silver ions may trigger apoptosis by stimulation of cytochrome c release from mitochondria. The present study explored the effect of AgNO~3~ on eryptosis, the suicidal death of erythrocytes, cells devoid of mitochondria. Similar to apoptosis of nucleated cells, eryptosis is characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine exposure at the cell surface. Eryptosis is triggered by energy depletion, cellular depletion of nitric oxide (NO) and activation of protein kinase C (PKC). Phosphatidylserine exposure was determined by annexin V‐binding, cell volume by forward scatter, cellular ATP by a luciferin–luciferase assay kit, and hemolysis by photometry. A 48 h exposure to AgNO~3~ (≥100 nm) but not to NaNO~3~ significantly enhanced the percentage of annexin V‐binding cells, slightly but significantly decreased forward scatter and significantly decreased cytosolic ATP. Furthermore, inhibition of PKC by staurosporine and donation of NO by sodium nitroprusside significantly blunted silver‐induced eryptosis. In conclusion, AgNO~3~ triggers cell membrane scrambling, an effect attributed to ATP depletion, PKC activation and decrease of cellular NO. Copyright © 2009 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Azathioprine-induced suicidal erythrocyt
Azathioprine-induced suicidal erythrocyte death
✍ Corinna Geiger; Michael Föller; Klaus R. Herrlinger; Florian Lang 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 321 KB

Background: Azathioprine is widely used as an immunosuppressive drug. The side effects of azathioprine include anemia, which has been attributed to bone marrow suppression. Alternatively, anemia could result from accelerated suicidal erythrocyte death or eryptosis, which is characterized by exposure

Tin triggers suicidal death of erythrocy
Tin triggers suicidal death of erythrocytes
✍ Tan Thanh Nguyen; Michael Föller; Florian Lang 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 324 KB

## Abstract Suicidal erythrocyte death or eryptosis is characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine (PS) exposure at the erythrocyte surface. Triggers of eryptosis include increase in cytosolic Ca^2+^ activity, formation of ceramide and energy depletion. Exce

Rapid astrocyte death induced by transie
Rapid astrocyte death induced by transient hypoxia, acidosis, and extracellular ion shifts
✍ Alexander Bondarenko; Mitchell Chesler 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 166 KB

## Abstract Death of astrocytes requires hours to days in injury models that use hypoxia, acidosis, or calcium paradox protocols. These methods do not incorporate the shifts in extracellular K^+^, Na^+^, Cl^−^, and Ca^2+^ that accompany acute brain insults. We studied astrocyte survival after expos

Calcium dependence of rapid astrocyte de
Calcium dependence of rapid astrocyte death induced by transient hypoxia, acidosis, and extracellular ion shifts
✍ Alexander Bondarenko; Mitchell Chesler 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 140 KB

## Abstract Exposure to hypoxic, acidic, ion‐shifted Ringer (HAIR) for 15–40 min has been shown to cause rapid astrocyte death upon reperfusion with normal media. The ion shifts of the HAIR solution included a rise in extracellular K^+^ (e.g., [K^+^]~o~) and a fall in [Na^+^]~o~, [Cl^−^]~o~, and [C

Liquid secondary ion mass spectral study
Liquid secondary ion mass spectral study of fatty acid methyl esters: silver cationization and collision induced dissociation studies
✍ K. Suma; N. Prasada Raju; M. Vairamani 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 180 KB 👁 2 views

A preliminary investigation of silver ion interactions with saturated and unsaturated fatty acid methyl esters under liquid-assisted secondary ion mass spectral conditions is reported. Collision induced dissociation studies of silver cationized species, [M + Ag] + , have shown regular charge remote