Silica-immobilized enzymes for multi-step synthesis in microfluidic devices

✍ Scribed by Heather R. Luckarift; Bosung S. Ku; Jonathan S. Dordick; Jim C. Spain

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 136 KB
• Volume: 98
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.21447

✦ Synopsis

Abstract

The combinatorial synthesis of 2‐aminophenoxazin‐3‐one (APO) in a microfluidic device is reported. Individual microfluidic chips containing metallic zinc, silica‐immobilized hydroxylaminobenzene mutase and silica‐immobilized soybean peroxidase are connected in series to create a chemo‐enzymatic system for synthesis. Zinc catalyzes the initial reduction of nitrobenzene to hydroxylaminobenzene which undergoes a biocatalytic conversion to 2‐aminophenol, followed by enzymatic polymerization to APO. Silica‐immobilization of enzymes allows the rapid stabilization and integration of the biocatalyst within a microfluidic device with minimal preparation. The system proved suitable for synthesis of a complex natural product (APO) from a simple substrate (nitrobenzene) under continuous flow conditions. Biotechnol. Bioeng. 2007;98: 701–705. © 2007 Wiley Periodicals, Inc.