Silica carriers for immobilization of thermostable α-amylase

✍ Scribed by E. Dobreva; V. Ivanova

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 329 KB
• Volume: 11
• Category: Article
• ISSN: 0138-4988
• DOI: 10.1002/abio.370110318

✦ Synopsis

The possibilities for immobilization of thermostable a-amylase from Baci2lus lichenijormis 44MBS2 on silica carriers activated b y different methods have been studied. Immobilization on Ti(1V)-activated GPG, Chromosorb P, quartz powder and pumice stone was sufficiently effective.

The preparation immobilized on CPG showed a shift in p H end temperature-profiles of enzyme action. They were found to be changed from p H 6,O-6,5 to p H 8,5 end from 90°C to SOOC, respectively, when compared to these parameters of the soluble enzyme. Immobilization leads t o enhancement of thermostability. Possibilities for batchwise use of the immobilized preparation were established.