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Silencing on the Spot. Induction and Suppression of RNA Silencing in the Agrobacterium-Mediated Transient Expression System

✍ Scribed by Johansen, L. K.


Book ID
118058246
Publisher
American Society of Plant Biologists
Year
2001
Tongue
English
Weight
741 KB
Volume
126
Category
Article
ISSN
0032-0889

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## Abstract RNA interference (RNAi) is a potent mechanism for local silencing of gene expression and can be used to study loss‐of‐function phenotypes in mammalian cells. We used RNAi to knockdown specifically the expression of choline acetyltransferase (ChAT), the enzyme of acetylcholine biosynthes