Significant role of intragraft lymphoid tissues in preventing insulin-dependent diabetes mellitus recurrence in whole pancreaticoduodenal transplantation
✍ Scribed by Masayuki Tori; Toshinori Ito; Takeyoshi Yumiba; Akira Maeda; Tsutomu Sawai; Masayuki Miyasaka; Hiroshi Kiyono; Hikaru Matsuda; Masumi Nozawa; Ryota Shirakura
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 103 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0738-1085
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✦ Synopsis
Graft recurrence of insulin-dependent diabetes mellitus (IDDM) was examined. Islet transplantation or pancreasalone transplantation excluding the duodenum and peripancreatic lymph nodes was compared with whole pancreaticoduodenal transplantation. A Wistar Furth (WF; RT1 u , RT6.2) to major histocompatibility complex (MHC)-compatible diabetes-prone (DP; RT1 u , RT6.1 gene carrier)-biobreeding (BB) rat transplantation model was used. Only DP recipients that had been transplanted with whole pancreaticoduodenal grafts were free from IDDM recurrence (>60 days postgrafting) when treated with anti-intercellular adhesion moluecule-1 (ICAM)-1/leukocyte function-associated antigen-1 (LFA-1) monoclonal antibodies (mAbs). In the spleen cells of the DP rats that had accepted pancreatic grafts (60 days postgrafting), flow cytometric analysis showed that NKR-P1 + TCR␣ + (NKT) cells had proliferated markedly, with the proportion of 12.8 ± 1.7% in the total splenic T cells, most of which (86.2%) were derived from the donor (RT6.2 + ). By enzyme-linked immunonosorbent assay (ELISA), serum interferon gamma (IFN-␥) was not detected (<13 pg/ml) in all rats. However, interleukin-4 (IL-4) was detected as 158.8 ± 28.0 pg/ml in the nonrecurrent DP recipients. These data suggested that to prevent IDDM recurrence in the pancreatic graft, the lymphocytes in the pancreaticoduodenal grafts are necessary. Also, the donor-derived NKT cells might have some immunoregulatory functions with a Th2 deviation.