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Significant Effect of Linker Sequence on DNA Recognition by Multi-Zinc Finger Protein

✍ Scribed by Makoto Nagaoka; Wataru Nomura; Yasuhisa Shiraishi; Yukio Sugiura

Book ID
115587750
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
263 KB
Volume
282
Category
Article
ISSN
0006-291X

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## Abstract The sequence‐specific affinity chromatographic isolation of plasmid DNA from crude lysates of __E. coli__ DH5Ξ± fermentations is addressed. A zinc finger‐GST fusion protein that binds a synthetic oligonucleotide cassette containing the appropriate DNA recognition sequence is described. T