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Significance of serum level of 25-hydroxycholecalciferol in gastrointestinal disease

✍ Scribed by Schoen, Malcolm S. ;Lindenbaum, John ;Roginsky, Martin S. ;Holt, Peter R.

Book ID
112476189
Publisher
Springer-Verlag
Year
1978
Weight
547 KB
Volume
23
Category
Article
ISSN
0002-9211

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Rapid high-performance liquid chromatogr
Rapid high-performance liquid chromatographic method for the estimation of toxicological levels of 25-hydroxycholecalciferol (25-OH D3) in human serum
✍ Jacek Łukaszkiewicz; Kazimierz KozΕ‚owski; Roman Lorenc πŸ“‚ Article πŸ“… 1983 πŸ› John Wiley and Sons 🌐 English βš– 281 KB

A reversed-phase HPLC method for determination of 25-hydroxycholecalciferol (25-OH D3) in serum samples is described. The method involves extraction of 25-OH D, with hexane, followed by differential resolubilization in acetonitrile. The sample is then applied directly on the CI8 bonded phase column