Significance of in vitro attachment of human colon cancers to extracellular matrix proteins in experimental and clinical liver metastases

✍ Scribed by Toshiharu Furukawa; Masahiko Watanabe; Dr. Tetsuro Kubota; Suguru Kase; Shin Fujita; Takaaki Yamamoto; Yoshiro Saikawa; Tsong-Hong Kuo; Hirokazu Tanino; Naoto Kurihara; Yukio Kawano; Kiyoshi Kawamoto; Akihiko Suto; Tatsuo Teramoto; Kyuya Ishibiki; Masaki Kitajima

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 566 KB
• Volume: 53
• Category: Article
• ISSN: 0022-4790
• DOI: 10.1002/jso.2930530105

✦ Synopsis

The attachment of 7 human colon cancer lines transplantable into nude mice, and primary tumors and liver metastases from 30 patients with colon cancer to 4 extracellular matrix proteins (EMPs)-Matrigel, laminin, fibronectin, and type IV collagen-was evaluated using the 3-(4,S-dimethylthiazol-2-yl)-2,S-diphenyl-2H (MTT) assay. Cancer cells from the 4 established tumor lines which produced experimental liver metastases in vivo showed significantly greater attachment to each EMP than those from the other 3 tumor lines which did not. Although there were no significant differences between attachment to EMPs of cancer cells from 1. 5 clinical primary tumors with liver metastases and those without, attachment to each EMP of cells derived from liver metastases was significantly greater than that of the cells from the corresponding primary tumors in 8 cases for which liver metastases and primary tumors were examined simultaneously. Attachment to EMPs, which could be determined simply and rapidly using the MTT assay, is thus considered a significant factor in experimental and clinical liver metastases of human colon cancers.