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Significance of IgG and IgM HCV antibody secretion in vitro in patients with chronic hepatitis C: Correlation with disease activity and response to interferon-α

✍ Scribed by Hanns Löhr; Christopher Nagel; Hans-Peter Dienes; Barry Simpson; Gerd Michel; Bernd Goergen; Karl-Hermann Meyer Zum Büschenfelde; Guido Gerken

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
703 KB
Volume
20
Category
Article
ISSN
0270-9139

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✦ Synopsis

Hepatitis C virus antibodies are found in the serum of most patients with chronic hepatitis C. However, the significance of the humoral response is still uncertain. In this study, in uitro IgG and IgM anti-hepatitis C virus secretion by peripheral blood mononuclear cells of patients with chronic hepatitis C was analyzed. Peripheral-blood mononuclear cells from 21 of 36 patients (58.3%) secreted IgG anti-hepatitis C virus in uitro, as demonstrated with anti-hepatitis C virus-specific enzyme immunoassays and recombinant immunoblot assays. Ten of the 36 patients (27.8%) showed both IgG and IgM anti-hepatitis C virus core in U ~~F O .

In 9 of these 10 patients, IgM anti-hepatitis C virus was also detected in serum. Patients with in uitro IgM or IgG anti-hepatitis C virus secretion had higher ALT levels in serum than did patients without such secretion in vitro (99.5 2 22.1 and 85.6 & 34.4 vs. 38.1 37.4 UL; p < 0.0001, p < 0.001). Furthermore, with a histology activity score it was demonstrated that patients with in uitro IgM or IgG HCV antibodies (or both) had more severe chronic active hepatitis than did patients without in uitro hepatitis C virus antibody secretion (p < 0.01). To analyze the therapy outcome, we included in this study 18 patients who had received interferon-a previously. Seven of eight in uitro hepatitis C virus antibody-positive patients were nonresponders, whereas the in uitro hepatitis C virus antibody-negative patients were mostly complete therapy responders (8 of 10). The follow-up study of eight patients with chronic hepatitis C after the beginning of therapy revealed that interferon-a decreases the in uitro humoral response to hepatitis C virus in treat-

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