Significance of fresh weight to dry cell weight ratio in plant cell suspension cultures
β Scribed by In-Suk Park; Dong-II Kim
- Book ID
- 104644195
- Publisher
- Springer-Verlag
- Year
- 1993
- Tongue
- English
- Weight
- 339 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0951-208X
No coin nor oath required. For personal study only.
β¦ Synopsis
The ratio of fresh weight to dry cell weight (FW/DCW), an index of cell water content, is important in determining the theoretical maximum cell concentration in high density culture of plant cell suspensions. Theoretical maximum cell density for Tbalictrum rirgosum was estimated to be as high as 137.2 g/l. The FWlDCW values were found to vary from species to species and it was related to medium osmolarity. When the cells were placed in a high osmolarity environment, decrease of FWlDCW was very rapid, taking place within 24 hours.
π SIMILAR VOLUMES
A simple apparatus was designed to allow sedimentation of plant cells grown in batch suspensions in Erlenmeyer flasks. After sedimentation the height of the cell mass along the glass wall was measured with a ruler fixed in the apparatus. The cell volume after sedimentation, calculated from this heig