Significance of cytomegalovirus (CMV)-pp65 antigenemia in the diagnosis of CMV disease in human immunodeficiency virus-infected patients

✍ Scribed by Reynes, Jacques; Montes, Brigitte; Atoui, Nadine; Segondy, Michel

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 434 KB
• Volume: 49
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/(sici)1096-9071(199607)49:3<195::aid-jmv6>3.0.co;2-#

✦ Synopsis

To establish the diagnostic value of the cytomegalovirus (CMVbpp65 antigenemia in CMV disease occur ri ng i n h u ma n i m m u n odef iciency (HIV)-infected patients, CMV-pp65 antigen in polymorphonuclear leukocytes (PMNLs) was assayed in 373 samples from 138 randomly included patients followed up for symptomatic HIV-1 infection and the correlation between CMV-pp65 antigenemia and diagnosis of CMV disease was investigated. Thirty-seven CMV disease episodes were observed in 30 patients and 89.2% of these episodes were associated with a positive CMV-pp65 antigenemia. In contrast, 94% of the patients negative for CMV-pp65 antigenemia remained free of CMV disease. Patients with CMV disease had significantly higher levels of CMV-pp65 antigenemia than CMV disease-free patients (695 positive cells/2 x lo5 PMNLs vs. 28 positive cells/2 X lo5 PMNLs). The positive and negative predictive values of the test were 45% and 94%, respectively, but were 93% and 8O%, respectively, when a CMV-pp65 antigenemia level of >I00 positive cells/2 X lo5 PMNLs was taken into consideration. These results indicate that the CMV-pp65 antigenemia assay is useful for the diagnosis and monitoring of CMV disease in HIV-infected patients. o 1996 Wiley-Liss, Inc.