𝔖 Bobbio Scriptorium
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Significance of Con A binding sites in the conjugation ofEuplotes vannus

✍ Scribed by Lueken, W. ;Oelgemöller, B.

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
640 KB
Volume
236
Category
Article
ISSN
0022-104X

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✦ Synopsis

Aggregations of Con A binding sites that occur on the surface of preconjugant cells have been studied in order to reveal their possible functions in the conjugation process. By means of a newly adapted horseradish peroxidase method, they can be visualized in distinctly shaped oval fields (OF) as soon as 10-15 min after mixing cells of opposite mating types. Cycloheximide (CHX) prevents establishment of new OFs but does not effect preexisting ones. In the absence of previously developed OFs, cells cannot form pairs. However, even if OFs are fully present, pairing still can be inhibited by CHX, high K + concentrations ( 2 60 mmolAiter), or the K+-channel blocker tetraethylammonium (TEA, 10 mmolAiter). A doublet cell with OFs on both halves 'As for the taxonomic status, see Corhss and Daggett ('83).

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