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Significance of amino groups of phosphatidylethanolamine in phospholipid peroxidation of mixed liposomes

✍ Scribed by Jin-Ye Wang; Zheng-Yu Wang; Tsutomu Kouyama; Toshiyuki Shibata; Tatzuo Ueki

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
486 KB
Volume
71
Category
Article
ISSN
0009-3084

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✦ Synopsis

The effect of distribution of phosphatidylethanolamine (PE) in multilamellar vesicles (MLV) on their peroxidative susceptibility was studied. Liposomes containing various ratios of L-alpha-dilinoleoyl phosphatidylcholine (DiLinPC) and L-alpha-dilinoleoyl phosphatidylethanolamine (DiLinPE) were peroxidized by ferrous ion or a water-soluble radical initiator 2,2'-azobis (2-amidino-propane) dihydrochloride (AAPH). The oxygen consumption rate was compared with the formation kinetics of fluorescent products. Inclusion of PE in liposomes was found to accelerate the Fe(2+)-dependent peroxidation. Modification of the amino group of PE with 2,4,6-trinitrobenzenesulfonic acid (TNBS) inhibited Fe(2+)-induced oxygen consumption in the late stage where fluorescent substances formed. A possible role of the amino group of PE on lipid peroxidation is discussed, especially in terms of its reactivity with oxidation products and subsequent formation of fluorescent substances.

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