Signal turn-on probe for nucleic acid detection based on 19F nuclear magnetic resonance
✍ Scribed by Takashi Sakamoto; Yu-ki Shimizu; Jun Sasaki; Hikaru Hayakawa; Kenzo Fujimoto
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 420 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0960-894X
No coin nor oath required. For personal study only.
✦ Synopsis
To image gene expression in vivo, we designed and synthesized a novel signal turn-on probe for 19 F nuclear magnetic resonance (MR) imaging based on paramagnetic relaxation enhancement. The stem-loop structured oligodeoxyribonucleotide (ODN) having a molecular beacon sequence for point mutated K-ras mRNA was doubly labeled with bis(trifluoromethyl)benzene moiety and Gd-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid chelate moiety at the each termini of the ODN probe, respectively. We found that the 19 F MR signal of the bis(trifluoromethyl)benzene moiety tethered at the 5 0 termini of the probe turned on by the addition of complementary ODN. The probe has the potential to image gene expressions in vivo.