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Signal transduction and bacterial conjugation: characterization of the role of ArcA in regulating conjugative transfer of the resistance plasmid R1

✍ Scribed by Heimo Strohmaier; Rainer Noiges; Sabine Kotschan; Gary Sawers; Gregor Högenauer; Ellen L Zechner; Günther Koraimann


Book ID
115628402
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
341 KB
Volume
277
Category
Article
ISSN
0022-2836

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The origin of transfer (oriT) is the sequence within which conjugal transfer of plasmid DNA is initiated, and is absolutely required in cis for plasmid mobilization. We have cloned oriT from the 52 kb IncN plasmid R46 on a 600 bp fragment, and mapped the limits of the relevant sequence by deletion a