𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Signal Selection in High-Resolution NMR by Pulsed Field Gradients: II. The Design of Gradient Pulse Sequences

✍ Scribed by David J. Thomas; Lorenz Mitschang; Bernd Simon; Hartmut Oschkinat

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
158 KB
Volume
137
Category
Article
ISSN
1090-7807

No coin nor oath required. For personal study only.

✦ Synopsis

We describe a new and powerful computer program called TRIPLE_GRADIENT which calculates optimized pulsed field gradient sequences for specific coherence pathway selection or rejection. Sequences can be computed for gradient coils acting along one, two, or three perpendicular axes. The program is based on the computational minimization of a penalty function formed from the summed amplitudes of the unwanted signals. The underlying mathematical analysis makes use of a vectorial representation of the way in which a gradient sequence suppresses different signals. It is argued that experiments using well-calculated gradient sequences are quicker and generally perform better than those using extensive phase cycling, especially when suppressing extremely strong solvent signals, and it is shown that in many cases gradient experiments of optimal signal-to-noise ratio can be performed. These claims are illustrated by spectra obtained from an HQQC experiment.

πŸ“œ SIMILAR VOLUMES

Signal Selection in High-Resolution NMR
Signal Selection in High-Resolution NMR by Pulsed Field Gradients: I. Geometrical Analysis
✍ Lorenz Mitschang πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 English βš– 98 KB

A geometrical description for the selection of coherence transfer pathways in high resolution NMR by the application of pulsed field gradients along three orthogonal directions in space is presented. The response of the spin system is one point of the threedimensional Fourier transform of the sample

High-resolution NMR experiments which us
High-resolution NMR experiments which use frequency-selective R.F. Pulses in combination with magnetic field gradients
✍ Michael A. Bernstein; Laird A. Trimble πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 English βš– 324 KB

## Abstract Magnetic field gradients were incorporated into the selective COSY experiment. The new gradient‐selected experiment has the expected advantages of significantly improved spectral quality resulting from better artifact suppression and the capacity to acquire spectra using fewer scans. A

Efficient Simulation of Coherence Transf
Efficient Simulation of Coherence Transfer Pathway Selection by Phase Cycling and Pulsed Field Gradients in NMR
✍ Alexej Jerschow; Norbert MΓΌller πŸ“‚ Article πŸ“… 1998 πŸ› Elsevier Science 🌐 English βš– 347 KB

The selection of well-defined coherence transfer pathways is an essential feature of all but the simplest NMR and EPR pulse sequences. This selection can be achieved by phase cycling and by pulsed field gradients. The properties of the RF-pulses (flip angle, offset effects, inhomogeneity) and transp

The study of the conformation and intera
The study of the conformation and interaction of two tachykinin peptides in membrane mimicking systems by NMR spectroscopy and pulsed field gradient diffusion
✍ Xinfeng Gao; Tuck C. Wong πŸ“‚ Article πŸ“… 1999 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 225 KB πŸ‘ 2 views

Pulsed-field gradient diffusion has been used to study the binding of two tachykinin peptides, [Tyr 8 ]-substance P (SP) and [Tyr 0 ]-neurokinin A (NKA) to two membrane-mimicking micelles, dodecylphosphocholine, and sodium dodecylsulfate. The structure of these peptides bound to the micelles have al

Identification of bound waters in the so
Identification of bound waters in the solution structure of ribonuclease T1 using the double pulsed field gradient spin-echo NMR technique for selective water excitation
✍ Mitsuru Tashiro; Kazuo Furihata; Sakurako Shimotakahara; Heisaburo Shindo πŸ“‚ Article πŸ“… 2002 πŸ› John Wiley and Sons 🌐 English βš– 93 KB

## Abstract Novel pulse sequences incorporating the double pulsed field gradient spin‐echo technique are presented that have particular use in identifying macromolecular bound water. The use of these sequences is illustrated using ribonuclease T~1~. Five amide protons cross‐relaxing with bound wate