Signal-Enhanced Amperometric Immunosensor Based on Ferrocene-Branched Poly(allylamine)/Multiwalled Carbon Nanotubes Redox-Active Composite

✍ Scribed by Jian-Ding Qiu; He Huang; Ru-Ping Liang

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 503 KB
• Volume: 23
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.201100212

✦ Synopsis

Abstract

A signal‐enhanced immunosensor has been developed by self‐assembling Au NPs onto a ferrocene‐branched poly(allylamine)/multiwalled carbon nanotubes (PAA‐Fc/MWNTs) modified electrode for the sensitive determination of hepatitis B surface antigen (HBsAg) as a model protein. The formation of PAA‐Fc/MWNTs composite not only effectively avoided the leakage of Fc and retained its electrochemical activity, but also enhanced the conductivity and charge‐transport properties of the composite. Further adsorption of Au NPs into the PAA matrix provided both the interactive sites for the immobilization of hepatitis B surface antibody (HBsAb) and a favorable microenvironment to maintain its activity. Tests performed with this immunosensor showed a specific response to HBsAg in the range of 0.1–350.0 ng mL^−1^ with a detection limit of 0.03 ng mL^−1^.