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Sialic acids in human lymphocytes. Qualitative and quantitative alterations in cancer cases

✍ Scribed by Regina Holzhauser; Hans Faillard

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 538 KB
Volume: 183
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(88)80048-x

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✦ Synopsis

Sialic acids, hydrolyzed from human lymphocytes, were determined, in the nanomole range, with a modified form of the periodic acid-thiobarbituric acid assay and liquid chromatography. The l.c. separations were carried out with two different systems, firstly an Aminex HPX-72 S anion-exchange resin and a 0.15M ammonium sulfate mobile phase, and secondly an amine phase (5 microns) and an acetonitrile-phosphate buffer as mobile phase. The lymphocytes of cancer-stricken persons showed an evident rise of the sialic acid content, combined with a shift of the sialic acid distribution to higher O-acetylated derivatives, as compared to the controls.

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